Stem Cell|
Research group for stem cells & tissue engineering The research group for stem cells and tissue engineering has been the first group developed in Stem Technology Company beginning its research work early in establishment of the company. The specialists working in this research group, mainly from basic medical sciences fields such as hematology, immunology, biochemistry, medical biotechnology and other specialties, are engaged in various fields related to stem cell research with proper organization. This group has close contacts with other research groups to achieve short and long term goals of the complex. Nanoscaffolds required for cell culture are assembled by biometrial and nanotechnology groups based on desired parameters, and are used for various transplantation practices after being evaluated through diverse tests for biological properties in laboratory environment. Collaboration of molecular biology and genetics engineering groups for molecular confirmation of the engineered tissues is essential and is an important strength point of this company to attain its goals. The most important research topics of this group are as follows: 1.Study of biology of stem cell types including embryonic stem cells, mesenchymal stem cells, unrestricted somatic stem cells, hematopoietic stem cells and tissue specific stem cells from various biological sources including umbilical cord blood, bone marrow and different tissues of human, mouse and rat.2. Bone tissue engineering aiming at applications in repair of bone injuries 3. Skin tissue engineering aiming at future applications in burns and cosmetic uses 4. Liver tissue engineering using concurrent use of stem cells and appropriate nanoscaffolds 5. Design and work with specific bioreactors for three dimensional culture, proliferation and differentiation of stem cells in controlled conditions 6. Three dimensional culture of stem cells and their differentiated products on biocompatible and biodegradable scaffolds made by biometrial and nanotechnology groups with trade mark NanoECM and other newly designed scaffolds and evaluation of the cell behavior 7. Nuclear transfer in mouse model as primary model to achieve cloning therapy 8. Production of transgenic mice with the ultimate goal of producing transgenic productsMicroRNA profiling technique MicroRNAs (miRNAs) are short (21–23 nucleotides) sequence-specific regulatory molecules found in all multi-cellular organisms. Over the last few years, several hundred conserved and nonconserved miRNAs have been identified and characterized in animals and plants using cloning and prediction strategies. Recent evidence indicates that they play important roles in a wide range of biological processes including developmental timing, differentiation, and growth control.Once identified, the temporal and spatial regulation of miRNA expression is generally investigated to better understand their biological function in vivo. To this end, numerous techniques for detecting miRNAs and other small RNAs have been developed including a variety of microarray-based and PCR-based approaches, rolling circle amplification, an ELISA-based assay, bead-based assays, single molecule detection, and the use of signal amplifying ribozymes. Many of these techniques require specialized and expensive equipment, and others involve multiple steps and an amplification reaction. To date, the most straightforward and widely used assay for small RNA detection has been traditional Northern blotting. Nevertheless, even with improvements in sensitivity provided by LNA substituted probes, Northern blotting requires relatively large amounts of starting material and involves multiple, timeconsuming handling steps. Therefore, we sought to develop an assay for small RNA detection that retained the simplicity of Northern blotting but eliminated its disadvantages. The developed assay takes advantage of liquid hybridization kinetics and circumvents the transfer, prehybridization, and washing steps required for Northern blotting. Tissue specific reporter vectors for stem cell studiesTissue specific expression vectors have several important applications both for research purposes and clinical use. In the field of stem cell biology tissue specific vectors expressing a reporter gene such as green fluorescent protein (GFP) could be used in monitoring of the differentiation processes. They have also many applications in controlled gene expression for therapeutic purposes in both cell and gene therapeutic procedures. Design and construction of tissue specific reporter vectors with several applications in stem cell research is the main goal of our research team. Our current project is construction of neuron specific reporter vectors for real time monitoring of the differentiation of both embryonic and adult stem cells into mature neurons. For designing our neuron specific reporter constructs, we have used the regulative elements in the promoter region of the well-known neuron specific genes. The resulting constructs are also applicable in development of brain targeting vectors for use in cancer gene therapy. Development of the Stem State Specific Reporter Vectors is the future direction of our research team. These vectors are very important research tools in studies and optimization of the conditions for stem cell isolation, culture and maintenance and in research studies for simulating the microenvironment of the stem cell’s niches. Services:
Masoud Soleimani Atashi Amir Ahmadbeigi Naser Gheisari Yousof This e-mail address is being protected from spam bots, you need JavaScript enabled to view it Nadri Samadstudent of ph.D in Nanomedicine Researcher s.nadri@stemcellstech.com This e-mail address is being protected from spam bots, you need JavaScript enabled to view it Soudi Sara student of ph.D in Immunology Ms.c. in Immunology Researcher m.hashemi@stemcellstech.com This e-mail address is being protected from spam bots, you need JavaScript enabled to view it Mohammad SalehiPh.D. Student of Anatomical Sciences M.Sc. in Histology Researcher m.salehi@stemcellstech.com This e-mail address is being protected from spam bots, you need JavaScript enabled to view it Mohammadian AliPh.D. Student of Biotechnology M.Sc. in Biotechnology Researcher a.mohammadian@stemcellstech.com This e-mail address is being protected from spam bots, you need JavaScript enabled to view it Kabiri Mahboobeh Ph.D. Student of Biotechnology M.Sc. in Biotechnology Researcher b.bakhshandeh@stemcellstech.com Seyed Jafari Oliaeenejad Ehsan Ph.D. Student of Biotechnology M.Sc. in Biotechnology Researcher e.seyedjafari@stemcellstech.com Bizhangi Khosro B.Sc. in Biology Researcher s.amini@stemcellstech.com Dinarvand Peyman Sadr Seyed Alireza This e-mail address is being protected from spam bots, you need JavaScript enabled to view it Babaei Farshad |
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